Pro-subtilis (Bacillus subtilis DE111®)
Pro-subtilis is a food supplement containing Bacillus subtilis DE111®, a well-researched probiotic strain that helps support normal digestive function and intestinal balance.
Pro-subtilis (Bacillus subtilis) DE111® supports the body’s natural recovery process, helping athletes prepare for training, competition, and optimal performance.
Bacillus subtilis DE111® is a scientifically studied probiotic strain shown to help maintain a balanced gut microbiota in young children.
Bacillus subtilis DE111® is a clinically studied probiotic that helps support your body’s natural defences and promotes everyday well-being.
By helping to maintain a healthy and balanced gut microbiota, Bacillus subtilis DE111® plays a key role in supporting normal immune function.
Pro-subtilis (Bacillus subtilis DE111®) is a scientifically studied probiotic that helps support a healthy balance of beneficial bacteria in the gut — an important foundation for digestive comfort and overall well-being.
This resilient strain is known for its ability to survive stomach acid and reach the intestines, where it helps maintain a healthy balance of beneficial bacteria and supports normal digestive processes.
The strain Bacillus subtilis DE111® has a lot of talents, which is why it has been nominated for "Microbe of the Year 2023."
Its role in improving gut function and supporting the immune system has made it a standout candidate in this prestigious category.
Clinical testing shows that the daily intake of Bacillus subtilis within the range of 1 to 5 billion CFU is just enough for its beneficial functions.
Furthermore, Bacillus subtilis DE111® promotes the growth of your own gut microflora Bifidobacterium species at a daily intake of 5 billion CFU.
Certain vitamins and minerals may interact with probiotics, potentially compromising their stability and colonization.
To ensure the maximum safety and effectiveness of the Bacillus subtilis DE111® probiotic strain, we have chosen to present it as a monoculture probiotic product.
In fact, Bacillus subtilis DE111® multiplies in the small intestine without the need for any prebiotics. Additionally, it produces short-chain fatty acids (SCFAs), which act as prebiotics for other beneficial bacteria in the gut.
For example, DE111® is clinically proven to stimulate the growth of your own gut's Bifidobacterium longum.
Each Pro-subtilis capsule is assured to contain a minimum of 5 billion CFU of Bacillus subtilis DE111® at the expiration date.
Storage stability is assessed in the United States.
Bacillus subtilis DE111® is one of the few probiotic strains that does not require refrigeration.
Bacillus family microbes are capable of forming spores, enabling them to endure challenging environmental conditions – like high temperatures or resisting gastric acid.
All probiotic strains, with almost no exceptions, are moisture sensitive.
Pro-subtilis probiotic is packaged in a special container designed to protect it from humidity.
Be sure to close the package tightly after use!
The ability to reach the intestine alive is a key characteristic of probiotic species.
Unlike some lactic acid bacteria such as Lactobacillus or Bifidobacterium, which may require specific conditions to remain viable, Bacillus subtilis forms a naturally resistant spore that helps it withstand challenging environments, including stomach acidity.
B. subtilis DE111® concentration in the small intestinal tract of healthy individuals with an ileostomy stoma. Vegetative DE111® (●), DE111® spores (■), and placebo (▲). Values are average concentrations (n = 11) ± standard deviation.
(Merritt & al., “Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract”, 2021)
Bacillus subtilis DE111® is a spore-forming probiotic strain that can survive passage through the digestive system.
Maintaining a balanced intestinal microbiota is important for digestive comfort and regularity.
A randomised, placebo-controlled human study has investigated the effects of Bacillus subtilis DE111® on digestive parameters in healthy adults.
Participants consuming DE111® showed differences in stool consistency and intestinal comfort compared with placebo under study conditions.
(Journal of Probiotics & Health, 2020)
A balanced intestinal microbiota plays an important role in overall digestive well-being.
Bacillus subtilis DE111® is a spore-forming probiotic strain that can survive passage through the digestive system and interact with other naturally occurring bacteria in the gut.
A randomised, placebo-controlled clinical study examined the effects of Bacillus subtilis DE111® on the gut microbiota of healthy adults.
Analysis of faecal samples showed changes in the relative abundance of several bacterial groups, including naturally present Bifidobacterium species.
(Merritt et al., Journal of Probiotics & Health, 2020)
The gut microbiota plays an important role in the normal function of the immune system.
A balanced composition of intestinal bacteria, including the ratio of Firmicutes to Bacteroidetes, is considered a marker of overall gut health.
A randomised, placebo-controlled clinical study investigated the effects of daily consumption of Bacillus subtilis DE111® (1 billion CFU) on immune and microbiota parameters in healthy adults.
The study observed changes in the distribution of certain immune-cell populations and microbial groups compared with placebo.
(Merritt et al., International Journal of Molecular Sciences, 2021)
Another study in children reported differences in gut-microbiota composition after eight weeks of DE111® consumption, including variation in several Bacteroidetes and Firmicutes genera associated with normal immune regulation.
(Paytuví et al., Frontiers in Microbiology, 2020)
Using a combination of in-vitro functional assays and genome analysis, researchers found that DE111® demonstrated the ability to survive harsh conditions such as those found in the GI tract, as well as pasteurization.
It also produces short-chain fatty acids, which support healthy lower GI function, as well as an increase in enzymatic activity (such as proteases, peptidases and esterases) and carbohydrate metabolism, pointing to its ability to promote a more efficient digestive process.
Additionally, researchers observed the strain’s ability to outcompete several types of unwanted bacteria associated with urinary, skin and intestinal flora imbalances.
Bacillus subtilis DE111® has been studied in active individuals
A clinical study entitled “Effects of Probiotic (Bacillus subtilis DE111®) Supplementation on Immune Function, Hormonal Status, and Physical Performance in Division I Baseball Players” was published in the journal Sports.
The research investigated daily consumption of Bacillus subtilis DE111® (1 billion CFU) during an eight-week training period in healthy athletes.
Results showed differences in certain physiological markers, including cytokine levels, compared with placebo.
These findings suggest that Bacillus subtilis DE111® may play a role in supporting normal post-exercise balance when combined with appropriate nutrition and training.
In a study involving female collegiate athletes during offseason training, researchers found that DE111® (1 billion CFU), in conjunction with adequate post-workout nutrition, can improve body composition and indices of athletic performance.
The results of the study showed that compared to placebo, the probiotic DE111® produced statistically significant improvements in the reduction of body fat percentage and a strong trend indicating improved performance of the deadlift exercise.
